Antibody/Protein Conjugation

Abnova Diagnostics offers high quality, efficient and cost-saving conjugation service between antibody/protein and fluorescent dyes, phycobiliproteins (RPE, APC, cl-APC), FITC, streptavidin, biotin and horseradish peroxidase (HRP). Since every antibody/protein has its own unique characteristics, conjugation needs to be optimized. 0.2mg of total antibody/protein of interest will be tested and HPLC analyzed prior to mass labeling. The tested sample will be mixed with mass conjugate product for final purification. We will complete the conjugation within 2-3 weeks from the time we receive the antibody/protein sample.

General Working Progress of Antibody Conjugation

  • Evaluation and Discussion of Conjugation Requirement and Service
  • Completion of the Custom Conjugation Specification (, )
  • Confirmation of Receipt of Antibody/Protein Sample
  • Conjugation Buffer Exchange
  • Antibody/Protein Concentration and Volume Analysis
  • Test-Scale Conjugation Optimization
  • Mass Antibody/Protein Labeling
  • Chromatographic Gel Purification
  • Spectrophotometer Measurement
  • COA, Packing, and Shipment

Table of Labeling Molecules and Excitation/Emission Wavelengths

Labeling Molecule Excitation Max (nm) Emission Max (nm)
RPE 495, 565 575
APC 650 660
cl-APC (PEAK) 495, 566 660
FITC 495 520

Special Notes:


  • The antibody/protein of interest should not contain albumin, gelatin, or other amine-containing stabilizers. Purification of antibody/protein from ascites fluid, serum, or cell culture medium or removal of stabilizer proteins prior to conjugation will result in additional cost to the customer.
  • Minimum quanity of antibody/protein conjugation is 0.1mg. Minimum concentration is 0.2mg/ml.

For any inquiry, please contact :

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