Circulating Fetal Nucleated Red Blood Cell Service

Non-invasive, circulating fetal nucleated red blood cell (fNRBC) enrichment, enumeration, and isolation for prenatal diagnostic management.

Abnova Diagnostics provides ISO15189 medical laboratory service to physicians and clinical researchers investigating circulating fetal nucleated red blood cell (fNRBC) in prenatal patients. Our capability is supported by a sophisticated platform for enrichment, enumeration, and cell isolation of highly pure fNRBCs. Such liquid biopsy is a revolutionary tool for the next generation, non-invasive prenatal diagnosis (NIPD) based on fetal cells and their complete chromosomal composition. Current non-invasive prenatal testing (NIPT) is based on fragmented circulating cell-free fetal DNA (cffDNA) analysis of the peripheral blood for trisomy 13, 18, and 21 only.

Specimen Requirement


  • Human whole blood: at least 7ml collected in ACD tube (yellow cap) at room temperature.
  • Shipment: sample should be kept in room temperature within 6 hours of collection and throughput transportation.
  • Time of testing: sample should be tested within 6 hours of collection to maintain cell freshness.

Cell Enrichment, Enumeration and Isolation


  • Sample preparation: whole blood fractionation via density gradient centrifugation.
  • Negative and positive enrichment: WBC depletion by negative antibody selection and fNRBC enrichment by positive affinity selection.
  • Cell enumeration: fNRBC biomarker identification via immunofluorescence and fluorescence in situ hybridization (FISH).
  • Cell isolation: fNRBC cell isolation via micromanipulator for whole genome amplification (WGA) and downstream analysis.

Analytical Methods


  • Immunofluorescence
  • Fluorescence in situ Hybridization
  • Array Comparative Genomic Hybridization (aCGH)
  • Next Generation Sequencing (NGS)

Deliverables (Sent Within 7 Working Days)


An Interpretative report of the fNRBC samples will be provided.

  • Number of fNRBCs enumerated based on biomarker identification.
  • Number of fNRBCs isolated for downstream analysis.
  • Additional FISH, STR, advanced molecular analysis per customer’s request.

Services


Items Package 1 Package 2 Package 3 Package 4
fNRBC Enumeration
fNRBC FISH      
fNRBC Cell Isolation    
fNRBC WGA    
fNRBC STR    
fNRBC aCGH      
fNRBC Next Generation Sequencing      
Total Price per Sample (USD/EUR) Inquire Inquire Inquire Inquire

CytoQuest™ CR system, SCx™ spiral chamber, TBx™ tension balancer, HBx™ micromixer, and TCx™ thermal control are trademarks and pending patents of Abnova Corporation.
Cell-Surface Vimentin (CSV) antibody is a pending MD Anderson patent which has been exclusively licensed to Abnova Corporation.
mutaFISH™ is a pending trademark and patent of Abnova Corporation.



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