Mouse & Rat Monoclonal Antibody

Abnova Diagnostics offers monoclonal antibody (Mab) development in mice and rats, with guaranteed success based on standard endpoints or customer's specific applications. Our high rate of success, short turn-around time and affordable cost are due to our proprietary hybridoma technology, state-of- the-art, specific pathogen free (SPF) facility, extensive experience in antibody production for over 10,000 gene targets, and flexibility to suit our customer's needs.

Monoclonal Antibody Development Workflow

  • Evaluation of Standard Antibody Production Service
  • Pre-Production Discussion: Custom Specification, Flowchart, Cost
  • Purchase Order
  • Production Specification Approval
  • Service Agreement and Production Specification Template (, )
  • Monoclonal Antibody Development and Progress Report
  • Monoclonal Hybridoma and Supernatant Delivery

Services Items Standard
Phase I Payment 1 (USD/EUR) Non-refundable startup fee Quote
Antigen Preparation Customer-supplied antigen (protein) *
Please quote if the customer requires antigen prepared by Abnova Diagnostics **
Customer-supplied antigen (peptide) *
Please quote if the customer require antigens prepared by Abnova Diagnostics**
Immunization Number of mice / rats immunized will be specified by the customer NA
Phase II Fusion
& Screening
Immunization of mice / rats and hybridoma fusion V
ELISA screen up to 1000 hybridoma clones V
Stabilization of up 10 positive clones V
Any mice / rats that shows a customer's required titer will be used to create ELISA positive monoclonal antibodies NA
Final Deliverables ELISA data and up to 10 clones of ELISA-positive hybridoma clones *** and culture supernatants (3ml/clone) V
The ELISA-positive monoclones will be delivered to customer in the format of 200 μg purified Mabs. Customer will test the supernatants on specific applications to determine the number of positive monoclones NA
Payment 2 (USD/EUR) Non-refundable Quote
Lead time (Month) 3~3.5
Phase III Hybridoma Delivery Customer will request hybridomas deemed suitable for customer's needs and pay Abnova Diagnostics for each hybridoma *** NA
Payment 3 (USD/EUR) Payment 3 is only paid upon hybridoma delivery NA
  Total Price (USD/EUR) Quote

*Antigen Requirement (provided by customer):
  • Protein immunization: 0.3~0.6mg
  • Peptide immunization: Conjugated-peptide (0.5mg) for immunization and Free-peptide (0.5mg) for screening step

**Immunogen Provided by Abnova Diagnostics:
  • Purified recombinant protein: additionalfee will be charged by Abnova Diagnostics.
  • Peptide: Peptide and Phosphopeptide: please refer to peptide synthesis service
  • Abnova Diagnostics may obtain from a source subject to customer's approval, and invoice the cost to the customer.

Lead time: ~1.5 month

***Cell Line Storage (3 vials/cell line/year) - Quote

Custom Mouse & Rat Monoclonal Antibody Service

For any inquiry, please contact :

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