mutaFISH™ Tissue Service

Single Cell, Single Molecule, DNA and RNA Mutation Detection at Single Nucleotide Resolution

Abnova Diagnostics provides an ISO15189 clinical laboratory service to physicians and clinical researchers investigating mutations in tissue samples. Our capability is powered by mutaFISH™ with integrated padlock probe and rolling circle amplification (RCA) technology for in situ, single cell, single molecule, DNA and RNA mutation at single nucleotide resolution. The ability to interrogate cellular heterogeneity in tissue has gained increase importance. While many technologies exist to address mutation changes, mutaFISH™ is the only precision and multiplexing tool to detect and quantify DNA and RNA changes while maintaining their spatial context. mutaFISH™ obviates the technical challenges of PCR and hybridization optimization required for efficient and target-specific analysis.

Specimen Requirement


  • Tissue specimen: prepare sample as fresh frozen tissue slide or formalin-fixed paraffin-embedded (FFPE) tissue slide. Provide a minimum of 4 tissue slides – one H&E stained slide with circled area of interest, and three blank slides for mutaFISH™ staining. Tissue section thickness should be at least 4 - 5 µm.
  • Shipment: ship fresh frozen tissue slide in dry ice. Ship H&E and FFPE tissue slides in 4°C or room temperature depending on its previously stored temperature.


Analytical Methods


  • Fresh frozen tissue mutation-specific Fluorescence In Situ Hybridization (Fresh Frozen tissue mutaFISH™)
  • Formalin-fixed paraffin-embedded tissue mutation-specific Fluorescence In Situ Hybridization (FFPE tissue mutaFISH™)


mutaFISH™ Probes vs Conventional DNA and RNA FISH Probes


Items mutaFISH™ Probe Conventional DNA FISH Probe Conventional RNA FISH Probe
Point Mutation Yes No No
Gene Amplification Yes Yes No
Gene Deletion Yes Yes No
Gene Translocation Yes Yes No
Gene Expression Yes No Yes


Deliverables (Sent Within 7 Working Days)


An interpretative report of mutaFISH™ tissue sample will be provided.

  • mutaFISH™ panel used to identify mutation changes
  • Additional protein biomarker staining per customer’s request


Services


Items Package 1 Package 2 Package 3 Package 4
Fresh Frozen Tissue mutaFISH™    
FFPE Tissue mutaFISH™    
Protein Biomarker Staining    
Total Price per Sample (USD/EUR) Inquire Inquire Inquire Inquire

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